Journal: Pharmacological research

Article Title: CircRNA CDR1as affects functional repair after spinal cord injury and regulates fibrosis through the SMAD pathway.

doi: 10.1016/j.phrs.2024.107189

Figure Lengend Snippet: Fig. 4. CDR1as regulates NSCs differentiation and microglia polarization. NSCs differentiation was identified by immunofluorescence. GFAP staining was used to detect astrocytes and MAP2 was used to detect nerve cells (A-B). The differentiation was further identified by PCR. The characteristics of nerve cells included MAP2, Nestin and TUJ1, and the characteristics of astrocytes were GFAP (C). iNOS (D) and Arg-1 (E) were detected by immunofluorescence after the BV2 model was established in vitro. Quantitative analysis of immunofluorescence staining (F). Expression of proinflammatory cytokines iNOS, IL-6, TNF-α (G) and anti-inflammatory cytokines Arg-1, CD206 and IL-4 (H) by PCR. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: The immunofluorescence steps of NSCs and BV2 differentiation are consistent with the identification of fibroblast, and primary antibodies were: inducible nitric oxide synthase (iNOS,6022, Proteintech, Rosemont, USA), Arginase-1 (Arg-1, 16001–1-AP, Proteintech), GFAP (3670, CST), and microtubule-associated protein 2 (MAP2, 17490–1-AP, Proteintech).

Techniques: Immunofluorescence, Staining, In Vitro, Expressing

Journal: Journal of Neurogastroenterology and Motility

Article Title: Molecular and Cellular Characteristics of the Colonic Pseudo-obstruction in Patients With Intractable Constipation

doi: 10.5056/jnm15048

Figure Lengend Snippet: Distribution of neuronal nitric oxide synthase positive (nNOS+) immunoreactivity. (A) nNOS+ cells were fewer in the transition zone (TZ) of the colonic pseudo-obstruction (CPO) colons, which appeared to be relatively dense aboral to the TZ region. (B) Comparison of nNOS+ cell counts between different regions indicates that loss of nNOS+ cells was less severe at locations further away from the TZ region. PD, proximal dilation; T, transverse colon; S, non-dilated sigmoid colon. Scale bar 40 μm. * P < 0.05, ** P < 0.01.

Article Snippet: Cryostat sections were cut to 20 μm, which were then pre-incubated in 1% bovine serum albumin for 1 hour to reduce non-specific binding and incubated at 4°C overnight with antibodies against neuronal nitric oxide synthase (nNOS) (1:500 dilution; BD Transduction Laboratories, San Jose, CA, USA), vasoactive intestinal peptide (VIP) (1:400 dilution; Millipore, Billerica, MA, USA), vesicular acetylcholine transporter (vAChT) (1:200 dilution; Phoenix Pharmaceuticals, Burlingame, CA, USA) and substance P (SP) (1:400 dilution; Millipore).

Techniques: